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KMID : 0380020160310040300
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Korean Journal of Biotechnology and Bioengineering
2016 Volume.31 No. 4 p.300 ~ p.311
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Characteristic as a Gene Delivery System of Water Soluble Chitosan Conjugated with Cationic Peptide
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Kim Young-Min
Kim Ji-Ho
Park Seong-Cheol
Park Yung-Hoon
Jang Mi-Kyeong
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Abstract
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Recently gene delivery has been designed newly using bioactive biomaterial and applied in the various field by many researchers. In this study, we proposed a new gene delivery system which has the capability of targeting effect in the specific tissue and remarkably enhanced transfection efficiency. We investigated 1H-NMR spectroscopy, particle size analyzer and gel retardation to confirm the correct preparation of gene delivery. Also, we identified the hemo-compatibility of gene delivery by hemolysis assay, non-cytotoxicity by MTT test and transfection efficiency. The uptake mechanism of the gene carrier was confirmed using inhibitor agent such as sodium azide, indomethacin, quercetin, colchicine, and chloropromazine. As a results, it was identified that gene carrier prepared by in this study entered in the cell by the microtubule-dependent, energy-dependent and clathrin-mediated endocytosis pathway.
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KEYWORD
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Gene carrier, Non-viral vector, Chitosan, transfection efficiency, Peptide
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